Identification of an ISSR Marker for 2-propenyl glucosinolate Content in Brassica juncea L. and Conversion to a SCAR Marker

Abstract

Research was undertaken to identify molecular markers using the ISSR technique for 2-propenyl glucosinolate in Brassica juncea. Bulked segregant analysis was used based on three bulks: (1) low 2-propenyl, low 3-butenyl glucosinolate, (2) high 2-propenyl glucosinolate, low 3-butenyl and (3) low 2-propenyl, high 3-butenyl glucosinolate. An ISSR marker was found to be tightly linked to high 2-propenyl glucosinolate. The ISSR fragment was cloned and sequenced and resulted in a 505 bp fragment bordered by the inverted SSR. An improved primer was designed based on the cloned sequence which resulted in a clear, simple to score, band associated with high 2-propenyl glucosinolate. The marker was screened against a range of canola and mustard quality B. juncea and was found to be widely applicable. The potential usefulness of the marker for canola quality and mustard B. juncea breeding programs is also discussed.