Identification of Allergic Epitopes of Soybean β-Conglycinin in Different Animal Species.

Yuan Zhao,Gaowa Naren,Nan Bao,Jianan Qiang,Guixin Qin,Mohammed Hamdy Farouk

doi:10.3389/fvets.2020.599546

Yuan Zhao, Gaowa Naren + Show 4 more

Open Access

https://doi.org/10.3389/fvets.2020.599546

Copy DOI

Abstract

Soybean can cause allergy in both humans and animals. The herein study aims to identify the antigenic determinants (epitopes) of β-conglycinin that lead to allergy in different animal species (swine, bovine, and rats). The epitopes of β-conglycinin were identified through co-immunoprecipitation and mass spectrometry. The binding abilities of seven identified epitope peptides to allergic sera of three animal species were compared by ELISA and dot-blot techniques. Some epitope peptides could be recognized by the three animal allergic sera, while most epitopes showed some differences in binding abilities to the different animal sera. The strongest reaction using swine sera was detected with peptides α2, β2, and β3, but the biggest sensitive regions for bovine and rats were peptides α2, β1, and β4. Most epitopes of β-conglycinin exhibited different binding abilities to the three animal sera, in which the biggest sensitive regions were peptides α2, β2, and β3 for swine, but peptides α2, β1, and β4 were detected for bovine and rats.

Highlights

Soybean is an important source of protein for both animals and humans worldwide
The allergy-causing epitopes of the α, α′, and β-subunits of βconglycinin were identified in different animal species by allergic sera IgG in this study, because the standard IgE in animals and anti-swine/bovine IgE antibodies are not available [30]
Sera IgG binding has been successfully employed by many researchers in allergenicity study using different animal models [29, 31,32,33]

Summary

Introduction

Soybean is an important source of protein for both animals and humans worldwide. The raw and processed forms of soybean represent ∼63% of all protein sources. Such forms are commonly used as protein ingredients in human food and in different animal species feeds [1]. The applicable amount of soybean meal in animal feeds (pets, poultry, swine, and bovine diets) exceeds 75% [2]. Soybean protein induces an allergic response in some animal species, such as swine [4,5,6,7], bovine [8, 9], rats [10], and dogs [11]

Objectives

Methods

Findings

Conclusion