Identification of alleles at the gliadin loci Gli-U1 and Gli-M b 1 in Aegilops biuncialis Vis.

Abstract

The diversity of alleles of gliadin loci Gli-U1 and Gli-M(b) 1 was studied in the tetraploid species Aegilops biuncialis (UUM(b)M(b)). The collection of 41 Ae. biuncialis accessions and F2 grain obtained from five crossing combinations provides material used in this study. Gliadins were separated by electrophoresis in polyacrylamide gel conducted in the acidic medium. To determine genomic affiliation (Uor M(b)) of components of Ae. biuncialis gliadin pattern, accessions of Ae. umbellulata and Ae. comosa were analyzed. In Ae. biuncialis accessions, 14 alleles were identified at the locus Gli-U1 and 12 alleles, at the locus Gli-M(b) 1. The results testify to a markedly high degree of allele diversity at major gliadin-coding loci of chromosomes belonging to Ae. biuncialis homeologous group 1.