Abstract
AFLP and bulked segregant analysis were used to identify molecular markers linked to resistance of cowpea [Vigna ungiculata (L.) Walp.] to parasitism by Striga gesnerioides (Willd.) Vatke. Segregation analysis of F2 progeny from a cross of Tvx3236, a Striga-susceptible line, with IT82D-849, a resistant cultivar, showed that resistance to S. gesnerioides race 1 from Burkina Faso was controlled by a single dominant gene, designated Rsg2–1. Three AFLP markers were identified that are tightly linked to Rsg2–1: E-AAC/M-CAA300 (2.6 cM), E-ACT/M-CAA524 (0.9 cM), and E-ACA/M-CAT140/150 (0.9 cM), which appears to be codominant. Segregation analysis of a different F2 population resulting from a cross of the Striga-susceptible line IT84S-2246–4 with Tvu 14676, a S. gesnerioides race 3 resistant line, showed that resistance to S. gesnerioides race 3 was also controlled by a single dominant gene, designated Rsg4–3. Six AFLP markers linked to Rsg4–3 were identified: E-ACA/M-CAG120 (10.1 cM), E-AGC/M-CAT80 (4.1 cM), E-ACA/M-CAT150 (2.7 cM), E-AGC/M-CAT150 (3.6 cM), E-AAC/M-CAA300 (3.6 cM), and E-AGC/M-CAT70 (5.1 cM). Segregation analysis of the E-AAC/M-CAA300 and E-ACA/M-CAG120 markers in recombinant inbred lines derived from IT84S-2049×524B determined that both are located within linkage group 1 of the cowpea genetic map. The identification of AFLP markers linked to Striga resistance provides a stepping stone for a marker-assisted selection program and the eventual cloning and characterization of the gene(s) encoding resistance to this noxious parasitic weed.
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