Abstract
The present investigation was undertaken to find molecular markers linked to leaf rust resistance genes, Lr9 and Kharchia local mutant KLM4-3B. Preliminary AFLP analysis was carried out with different stocks, a survey of primer combinations with different selective nucleotide indicated that for each primer combination, the number of scorable loci ranged from 34 to 123. Only a limited primer combination used in the set of parental and near isogenic lines showed a high level of polymorphism for AFLP marker. Putative AFLP marker were found to be linked to Lr9, Lr19 and KLM4-3B. The alien genes were readily identified.
Highlights
Wheat exceeds every other grain crop in acreage and production and is, the most important cereal of the world
A limited primer combination used in the set of parental and near isogenic lines showed a high level of polymorphism for Amplified Fragment Length Polymorphism (AFLP) marker
One of the leaf rust resistance genes, Lr9 transferred from Aegilops umbellulata [15] located on chromosome 6BL, has no undesirable effect associated with it [16]
Summary
Wheat exceeds every other grain crop in acreage and production and is, the most important cereal of the world. It is imperative to stabilize the wheat production by reducing the losses due to various diseases including leaf rust, stem rust, yellow rust, Karnal bunt etc. One of the ways to develop varieties with durable rust resistance is to pyramid the genes for resistance in a single variety [10]. One of the leaf rust resistance genes, Lr9 transferred from Aegilops umbellulata [15] located on chromosome 6BL, has no undesirable effect associated with it [16]. This gene is effective against all the races of leaf rust currently prevalent in northern India. KLM4-3B as these genes provide resistance against most of the leaf rust pathotypes of the Indian subcontinent
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