Identification of Acetic Acid Bacteria Isolates from Home Made Fermentation Liquids by PCR

Abstract

In this study, polymerase chain reaction primers based on adh III sequence (GenBank accession no. AB264314) were designed for identification of Acetobocter strains and acetic acid bacteria (AAB) isolates. This primer set termed ADH 187/ADH 500 was successfully used for identification of 41 AAB strains of 79 isolates directly from homemade vinegar fermentation. Furthermore, the acetate oxidation ability was used for analysis of acetate oxidation ability. The results showed that the AAB305 strain could produce higher acetate content than other AAB isolates in Yeast Glucose Mannitol/Mg(superscript 2+) (YGM/Mg(superscript 2+)) medium supplemented with 7% ethanol and could be used for industrial vinegar production. In addition, these isolates with high acetate oxidation ability were identified as Acetobacter sp. by the sequencing of the 16S rDNA PCR amplification products.