Identification of a Zn2 ‐sensitive component of Ehrlich cell plasma membrane redox system by CHAPS‐agarose‐polyacrylamide electrophoresis and in situ staining of activity

Abstract

A procedure based on CHAPS-agarose-polyacrylamide electrophoresis and in situ staining of activity was used to detect a Zn(2+)-sensitive component of Ehrlich cell plasma membrane redox system. The procedure is so powerful that it allows to use crude plasma membrane fractions and can be easily adapted for use in an electrophoretic approach to the purification of this protein.