Identification of a transcription factor that functions downstream of corazonin in the control of desert locust gregarious body coloration

Abstract

Corazonin (Crz) is a neuropeptide that controls phase-dependent body color polyphenism in locusts. The Crz signaling pathway is responsible for the development of gregarious black patterns in nymphs and determination of the morphometric ratio F/C (F = hind femur length, C = maximum head width) in adults. However, little information is available regarding the mediator and effector proteins regulated by Crz. In this study, we identified a novel transcription factor, Loct, which functions downstream of Crz in Schistocerca gregaria and Locusta migratoria. In S. gregaria, we detected a variant of Loct lacking the N-terminal region. Protein–protein interaction assays showed that both the long and short Loct variants formed a complex with themselves. LOCT knockdown in gregarious nymphs reduced the intensity of their black patterning, but did not affect F/C ratios in adults. LOCT was exclusively expressed in the integument of gregarious nymphs, suggesting that Loct is involved in melanin production. In addition, we found that the melanization-associated protein Yellow (YEL) and the albino-related takeout protein (ALTO) are expressed in the integument and function downstream of Crz. However, Crz injection failed to influence LOCT, YEL, and ALTO expression. Therefore, additional factors probably cooperate with Crz to induce these genes. The gene expression profiles of YEL and ALTO in LOCT-knockdown nymphs suggest that Loct does not directly control the transcription of YEL or ALTO. In summary, we present a working model of the Crz pathway, which is active in crowded S. gregaria nymphs.