Abstract
Proximity labeling has been used to study protein-protein interactions and can also be used as a protein labeling tool. We developed a novel 14-amino acid peptide substrate for the proximity-labeling enzyme PafA. The N terminus of the peptide can be modified with biotin or fluorophores, which allows various chemical moieties to be ligated to the target protein. We used PafA-mediated peptide labeling to label antibodies with a biotin tag without affecting the antigen binding capacity of the antibody. Similar strategies can be used for other types of protein labeling in the future.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
