Abstract
5-Phospho-d-ribosyl-1-diphosphate (PRPP) synthase (PRS) catalyzes the biosynthesis of PRPP, which is an important compound of metabolism in most organisms. However, no PRS genes have been cloned, let alone studied for their biological function in rubber tree. In this study, we identify a novel protein (PRS4) that interacts in vivo with rubber tree anaphase promoting complex/cyclosome (APC/C) subunit 10 (HbAPC10) by yeast two-hybrid assays. PRS4 has been cloned from rubber tree and named as HbPRS4. Blastp search in the genome of Arabidopsis thaliana showed that HbPRS4 shared the highest similarity with AtPRS4, with 80.71% identity. qRT-PCR was used to determine the expression of HbPRS4 in different tissues and under various treatments. HbPRS4 was preferentially expressed in the bark. Moreover, the expression level of HbPRS4 was significantly induced by the proteasome inhibitor MG132 treatment, suggesting it might be regulated by the ubiquitin/26S proteasome pathway. The amount of HbPRS4 transcript was obviously decreased after mechanical wounding and abscisic acid (ABA) treatments, while a slight increase was observed at 24 h after ABA treatment. HbPRS4 transcript in the latex was significantly upregulated by ethephon (ET) and methyl jasmonate (MeJA) treatments. These results suggested that HbPRS4 may be a specific substrate of HbAPC10 indirectly regulating natural rubber biosynthesis in rubber tree.
Highlights
5-Phospho-D-ribosyl-1-diphosphate (PRPP) synthase (PRS) catalyzes the biosynthesis of PRPP, which is an important compound of metabolism in most organisms
This is the first report on the interaction of APC10 with PRS4, and HbPRS4 gene was first cloned from rubber tree
Phylogenetic analysis supported HbPRS4 as a Class II PRPP synthase (PRS) with a high degree of similarity with Spinacia oleracea SoPRS4 and Arabidopsis thaliana AtPRS4
Summary
5-Phospho-D-ribosyl-1-diphosphate (PRPP) synthase (PRS) catalyzes the biosynthesis of PRPP, which is an important compound of metabolism in most organisms. We identify a novel protein (PRS4) that interacts in vivo with rubber tree anaphase promoting complex/cyclosome (APC/C) subunit 10 (HbAPC10) by yeast two-hybrid assays. HbPRS4 transcript in the latex was significantly upregulated by ethephon (ET) and methyl jasmonate (MeJA) treatments These results suggested that HbPRS4 may be a specific substrate of HbAPC10 indirectly regulating natural rubber biosynthesis in rubber tree. AtPRS3 may play a novel role in providing PRPP to cellular metabolism under P starvation [12] These studies demonstrated that plant PRSs have a variety of functions, which are different from the bacteria and mammals’ PRSs. Rubber tree We identify a novel protein (ribose-phosphate pyrophosphokinase 4, PRS4) that interacts in vivo with the Hevea brasiliensis APC/C subunit 10 (HbAPC10) using the yeast two-hybrid system. The results in this study suggested that HbPRS4 may serve as a specific substrate of HbAPC10 indirectly regulating NR biosynthesis in rubber tree
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