Identification of a recessive gene YrZ15-1370 conferring adult plant resistance to stripe rust in wheat-Triticum boeoticum introgression line.

Minghu Zhang,Ting Peng,Dengcai Liu,Zhongwei Yuan,Xin Liu,Shunzong Ning,Lianquan Zhang,Bo Jiang,Xue Chen,Dongyu Liang,Lin Huang,Dinghao Wang,Hongyu Li,Ming Hao,Xuejiao Chen

doi:10.1007/s00122-021-03866-3

Minghu Zhang, Ting Peng + Show 13 more

Open Access

https://doi.org/10.1007/s00122-021-03866-3

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Abstract

A novel recessive gene YrZ15-1370 derived from Triticum boeoticum confers adult-plant resistance to wheat stripe rust. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most damaging diseases of wheat globally and resistance is the effectively control strategy. Triticum boeoticum Boiss (T. monococcum L. ssp. aegilopoides, 2n = 2x = 14, AbAb) accession G52 confers a high level of adult-plant resistance against a mixture of the Chinese prevalent Pst races. To transfer the resistance to common wheat, a cross was made between G52 and susceptible common wheat genotype Crocus. A highly resistant wheat-T. boeoticum introgression line Z15-1370 (F5 generation) with 42 chromosomes was selected cytologically and by testing with Pst races. F1, F2, and F2:3 generations of the cross between Z15-1370 and stripe rust susceptible common wheat Mingxian169 were developed. Genetic analysis revealed that the resistance in Z15-1370 was controlled by a single recessive gene, tentatively designated YrZ15-1370. Using the bulked segregant RNA-Seq (BSR-Seq) analysis, YrZ15-1370 was mapped to chromosome 6AL and flanked by markers KASP1370-3 and KASP-1370-5 within a 4.3cM genetic interval corresponding to 1.8Mb physical region in the Chinese Spring genome, in which a number of disease resistance-related genes were annotated. YrZ15-1370 differed from previously Yr genes identified on chromosome 6A based on its position and/or origin. The YrZ15-1370 would be a valuable resource for wheat resistance improvement and the flanking markers developed here could be useful tools for marker-assisted selection (MAS) in breeding and further cloning the gene.

Highlights

Common wheat (Triticum aestivum L.) is one of the most important staple cereal crops for mankind, providing approximately 19% of the food calories and over 20% of the protein consumed by the world population (Braun et al 2010)
The wheat-T. boeoticum introgression line Z15-1370 was crossed with common wheat Mingxian169 to develop F1, F2, and F2:3 populations, which were used in stripe rust resistance assessments and gene mapping
Mc-FISH and multicolor genomic in situ hybridization (mc-GISH) analysis indicated that the Z15-1370 has 42 chromosomes with 14 A-genome, 14 B-genome, and 14 D-genome chromosomes, respectively (Fig. 1a, b)

Summary

Introduction

Common wheat (Triticum aestivum L.) is one of the most important staple cereal crops for mankind, providing approximately 19% of the food calories and over 20% of the protein consumed by the world population (Braun et al 2010). Tritici, Pst) is one of the most serious fungal diseases affecting wheat production worldwide (Wellings 2011). Breeding resistant cultivars is the most economical and sustainable method for stripe rust control. Host resistance of wheat against Pst could be classified as either all-stage resistance (ASR) or adult-plant resistance (APR). The ASR confers high levels of resistance that is mostly race-specific and is vulnerably overcome by the emergence of new virulent races (Ellis et al 2014). APR generally provides a partial level of resistance that is non-race specific and, in some cases, has proven to be more durable than ASR (Ellis et al 2014; Fu et al 2009; Lagudah et al 2009)

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