Abstract
Eastern filbert blight (EFB) is a serious disease of hazelnut and threatens crop production in the Willamette Valley of Oregon. We have employed the use of the bulk segregant analysis method to screen RAPD primers for identification of a marker linked to a disease-resistance gene for this pathogen. Using 100 progeny from a modified backcross population segregating for resistance to the disease, two pooled DNA samples from 10 individuals of each phenotype were constructed. One-hundred 10-mer PCR primers were screened for bands present in the resistant pool, but absent in the susceptible pool. We identified one primer that produces a marker cosegregating with resistance and appears to be linked to the resistance gene (frequency of recombination = 0.17). This marker proved to be easily scoreable and reliably reproduced, even under varying amplification profiles. From these results, we have determined that this method may be suitable for identification of markers more closely linked to the resistance gene. Such a marker would be useful for early selection of seedlings having resistance to EFB.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
