Identification of a Proanthocyanidin from Litchi Chinensis Sonn. Root with Anti-Tyrosinase and Antioxidant Activity.

Matthew Saive,Thibaut Istasse,Chloé Maes,Manon Genva,Michel Frederich,Marie-Laure Fauconnier

doi:10.3390/biom10091347

Matthew Saive, Thibaut Istasse + Show 4 more

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https://doi.org/10.3390/biom10091347

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Journal: Biomolecules	Publication Date: Sep 21, 2020
Citations: 6	License type: CC BY 4.0

Affiliation: Gembloux Agro-Bio Tech, University of Liège

Abstract

This work follows an ethnobotanical study that took place in the island of Mayotte (France), which pointed out the potential properties of Litchi chinensis Sonn. roots when used to enhance skin health and appearance. Through in vitro testing of a crude methanolic extract, high anti-tyrosinase (skin whitening effect) and antioxidant activities (skin soothing effect) could be measured. HPLC successive bio-guided fractionation steps allowed the purification of one of the compounds responsible for the biological activities. The isolated compound was characterized by UV, IR, MS and 2D-NMR, revealing, for the first time in Litchi chinensis Sonn. roots, an A-type proanthocyanidin and thus revealing a consensus among the traditional use shown by the ethnobotanical study, in vitro biological activities and chemical characterization.

Highlights

An ethnobotanical study which took place in the island of Mayotte [1] led to the conclusion that many plants are used traditionally among inhabitants of the island to promote health and for use in dermatological applications
It is the first time that this class of compounds was highlighted in L. chinensis’ roots
The fact that L. chinensis roots are used in this study may raise questions in terms of the plant's conservation

Summary

Introduction

An ethnobotanical study which took place in the island of Mayotte [1] led to the conclusion that many plants are used traditionally among inhabitants of the island to promote health and for use in dermatological applications. Using indicators such as the Informant Agreement Ratio developed by Trotter & Logan, 1986 [2], species of interest were selected for further studies. In vitro biological activities measurements realized on several crude extracts of the selected plants revealed that Litchi chinensis Sonn. Genus only contains one identified species composed of three subspecies: L. chinensis subsp. Trohlelaf.loTwheerys aarreesfmunalcltgiooninaglly mfterffpEocareivhomlmneemnknaaliliqtnewigofu.dhhrsTeetofihtdeupmeem.siteTneloaidhtkslcpeep.ht.aeEorilcTovefirorhmeeuytendieestl.lnysiilfotssTactwshrephoemre,imehwfrsrieeoesuiatnossihrttttptsraasesoayhcnttriesaagemttpplrehyeaemredmnioanostierfostltrp,uossrrmeuohtenrusasoocenpscenntdaedgltodylcbfayxotytovthiwatneherprifloeteLuhurdsioontoecboudnihlyttic,aceatasmordevcwroeooebsesruryoltegfllodothalhaufsb.renttiyThhdnsheedaoneiLprooyloirfwatouacpspargheewdaihrgatfiecurraylaeitnslnireocapdhstnsiafgoovtotonfeheuicapenhnfleilgpndbyririrfqcmrnoooauuopamreswapleugrlagosiaadgeoonthdaitidonsty. ngs ha(Fviegufirber1o)ufsouronodtsat(Fdiegputhreb1e)twfoeuennd0 aant dde6p0tchmb[e4t,w10e,1e1n].0 and 60 cm [4,10,11]

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