Abstract
Studies involving dissolution and methylation procedures, infrared spectroscopy, and 14C analyses were made on hymatomelanic acids extracted from forested and grassland soils. Methylated and nonmethylated hymatomelanic acids were found to possess similar infrared absorption characteristics. 14C-labelled ethanol extraction of suitable soil organic matter preparations yielded hymatomelanic acid which was nonradioactive. This indicated that the ester linkage was not an artifact of extraction with ethanol. By means of dissolution and reprecipitation, hymatomelanic acid was separated into fraction A, with humic acid characteristics, and fraction B, a polysaccharide. It was concluded that hymatomelanic acid is a naturally occurring ester compound composed of humic substances in ester linkages with polysaccharides.
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