Abstract
Tuberculosis (TB) is a major comorbidity in HIV patients as well as a serious co-epidemic. Traditional detection methods are not effective or sensitive for the detection of Mycobacterium tuberculosis at the early stage. TB has become a major cause of lethal on HIV patients. We employed isobaric tags for relative and absolute quantitation (iTRAQ) technology to identify the different host responses in HIV-noTB and HIV-TB patients’ sera. Given the diversity of HIV subtypes, which results in a variety of host responses in different human populations, we focused on the Chinese patients. Of the 25 proteins identified, 7 were increased and 18 were decreased in HIV-TB co-infected patients. These proteins were found to be involved in host immune response processes. We identified a candidate protein, endoglin (ENG), which showed an 4.9 times increase by iTRAQ and 11.5 times increase by ELISA. ENG demonstrated the diagnostic efficacy and presented a novel molecular biomarker for TB in HIV-infected Chinese patients. This study provides new insight into the challenges in the diagnosis and effective management of patients with HIV-TB.
Highlights
Tuberculosis (TB), a bacterial infectious disease, is a major comorbidity in HIV-infected individuals, many of whom suffer from acquired immunodeficiency syndrome (AIDS) (Harries, 1990; Getahun et al, 2010; Hickey et al, 2015; Ai et al, 2016)
By comparing HIV-infected patients and HIV-TB co-infected patients, we identified 25 proteins, including 7 were increased and 18 were decreased in HIV-TB co-infected patients that indicated a set of host response proteins as candidate biomarkers for the detection of TB in HIV patients in China
Co-infection of Mycobacterium tuberculosis (Mtb) and HIV is a tremendous threat for HIV patient due to the widespread distribution of Mtb and high risk of death
Summary
Tuberculosis (TB), a bacterial infectious disease, is a major comorbidity in HIV-infected individuals, many of whom suffer from acquired immunodeficiency syndrome (AIDS) (Harries, 1990; Getahun et al, 2010; Hickey et al, 2015; Ai et al, 2016). Studies have revealed that mycobacteria can more infect individuals with HIV infection than those (Pedersen et al, 1997; Corbett et al, 2003). These findings suggest a mutualistic relationship between the two pathogens. TB in HIV-infected patients is a major issue in the field of infectious diseases
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