Abstract
Rice is the most important staple food crop, and it feeds more than half of the world population. Brown planthopper (BPH) is a major insect pest of rice that causes 20–80% yield loss through direct and indirect damage. The identification and use of BPH resistance genes can efficiently manage BPH. A molecular marker-based genetic analysis of BPH resistance was carried out using 101 BC1F5 mapping population derived from a cross between a BPH-resistant indica variety Khazar and an elite BPH-susceptible line Huang–Huan–Zhan. The genetic analysis indicated the existence of Mendelian segregation for BPH resistance. A total of 702 high-quality polymorphic single nucleotide polymorphism (SNP) markers, genotypic data, and precisely estimated BPH scores were used for molecular mapping, which resulted in the identification of the BPH38(t) locus on the long arm of chromosome 1 between SNP markers 693,369 and id 10,112,165 of 496.2 kb in size with LOD of 20.53 and phenotypic variation explained of 35.91%. A total of 71 candidate genes were predicted in the detected locus. Among these candidate genes, LOC_Os01g37260 was found to belong to the FBXL class of F-box protein possessing the LRR domain, which is reported to be involved in biotic stress resistance. Furthermore, background analysis and phenotypic selection resulted in the identification of introgression lines (ILs) possessing at least 90% recurrent parent genome recovery and showing superior performance for several agro-morphological traits. The BPH resistance locus and ILs identified in the present study will be useful in marker-assisted BPH resistance breeding programs.
Highlights
Rice is an essential cereal crop that feeds more than 3.5 billion people globally
Besides the direct damage caused by brown planthopper (BPH), it causes indirect damage by transmitting viruses that results in a yield penalty (Sogawa1982; Cha et al 2008; Cabauatan et al 2009)
In order to study the mode of segregation of the BPH resistance derived from Khazar, genetic analysis was carried out
Summary
Rice is an essential cereal crop that feeds more than 3.5 billion people globally. There is a need to increase rice production by up to 42% by 2025 to feed the everincreasing population (Seck et al 2012; Ray et al 2013). About 52% of world rice production is lost annually because of living organisms, of which approximately 21% alone is contributed by the incidence of insect pests (Khush 1979; Sogawa et al 2003). Among these insect pests, brown planthopper (BPH; Nilaparvata lugens Stal.) is one of the major devastating sap-sucking pests, prevalent throughout the rice-growing countries of Asia (Normile 2008). BPH causes significant damage to the rice crop by sucking sap from the xylem and phloem tissues, which leads to ‘‘hopperburn’’ and, eventually complete yield loss. Besides the direct damage caused by BPH, it causes indirect damage by transmitting viruses (rice grassy stunt virus and ragged stunt virus) that results in a yield penalty (Sogawa1982; Cha et al 2008; Cabauatan et al 2009)
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