Year-end Sale % OFF 
Abstract
Background Genomic imprinting is the epigenetic process by which a subset of genes is expressed from only one allele based on the parent of origin. To date there are approximately 150 confirmed autosomal imprinted loci in the mammalian genome. The epigenetic hallmark of an autosomal imprinted locus is the presence of differential methylation at CpG islands otherwise known as a DMR[1]. Imprint maintenance at autosomal loci is accomplished through specific histone modifications[2]. In 2005, Raefski et.al. found the first X-linked imprinted locus, Xlr3/4 of which three paralogs, Xlr3b/4b/4c, were maternally expressed and paternally silenced in mouse neonatal brain[3]. Here we use a series of techniques to uncover the imprinting mechanism at the Xlr locus using known autosomal imprinting mechanisms as a model.
Highlights
Genomic imprinting is the epigenetic process by which a subset of genes is expressed from only one allele based on the parent of origin
We show that there is no differential methylation at the Xlr3/4 locus between 39, Xm and 39XP samples
Potential candidate regions for differential methylation according to MeDIP assays were disproven by bisulfite sequencing of CpG islands within those regions
Summary
Genomic imprinting is the epigenetic process by which a subset of genes is expressed from only one allele based on the parent of origin. To date there are approximately 150 confirmed autosomal imprinted loci in the mammalian genome. The epigenetic hallmark of an autosomal imprinted locus is the presence of differential methylation at CpG islands otherwise known as a DMR[1]. Imprint maintenance at autosomal loci is accomplished through specific histone modifications[2]. In 2005, Raefski et al found the first X-linked imprinted locus, Xlr3/4 of which three paralogs, Xlr3b/4b/4c, were maternally expressed and paternally silenced in mouse neonatal brain[3]. We use a series of techniques to uncover the imprinting mechanism at the Xlr locus using known autosomal imprinting mechanisms as a model
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
