Abstract
Peritrophic membranes (PMs) are composed of proteins, proteoglycans and chitin that play important roles in the structural formation and function of the PM. This study identified and characterized a new chitin binding protein named HpCBP45 by immunoscreening of the Holotrichia parallela larvae midgut expression library. The predicted amino acid sequence indicates that it contains eight tandem chitin binding domains belonging to the peritrophin-A family. The HpCBP45 protein was expressed as a recombinant protein in the yeast Pichia pastoris and chitin binding assay demonstrated that recombinant HpCBP45 protein could strongly bind to chitin. qRT-PCR analysis showed that HpCBP45 was mainly localized in the midgut, further confirming the H. parallela PM belongs to Type I PM. The discovery and characterization of the peritrophic membrane protein HpCBP45 provides a basis for the further investigation of its biochemical and physiological functions in H. parallela.
Highlights
In many insects, the midgut epithelium is lined with a semi-permeable structure called the peritrophic membrane (PM) or peritrophic matrix, and the functions of this semi-permeable membrane are crucial for the protection of the midgut epithelium from mechanical damage by food particles, bacterial damage and parasite invasion [1]
A Holotrichia parallela midgut cDNA expression library was screened with specific polyclonal antibody against PM proteins
In H. parallela larvae, the HpCBP45 was abundant in the midgut, suggesting that HpCBP45 was secrected by the whole midgut epithelium, which confirmed that the H. parallela PM belonged to the TypeIPM
Summary
The midgut epithelium is lined with a semi-permeable structure called the peritrophic membrane (PM) or peritrophic matrix, and the functions of this semi-permeable membrane are crucial for the protection of the midgut epithelium from mechanical damage by food particles, bacterial damage and parasite invasion [1]. Type II PM is synthesized as a continuous sleeve from the cell of the cardia in the anterior midgut of the insect [8], and it has been found in Dermaptera, Isoptera, Embiodea, and the larvae of Diptera [10]. The PM composed of proteins, proteoglycans and chitin, which together orchestrate the robust structure as well as the protective and semi-permeable functions of the matrix [11]. We identified and characterized a new PM protein HpCBP45 from Holotrichia parallela larvae by cDNA cloning. This protein possesses eight chitin-binding domains related to the peritrophin-A domain and its chitin-binding activity has been tested
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