Abstract
An EcoRI DNA fragment of 0.5 kb specifically stimulating levansucrase synthesis was cloned in Bacillus subtilis. Transcriptional stimulation of levansucrase expression was observed after induction by sucrose when the sequence was present on a multicopy plasmid in B. subtilis. The nucleotide sequence of the DNA fragment was determined. The involvement of a putative peptide is discussed. This fragment located on the chromosomal map of B. subtilis near the dal locus defines a new locus, sacV, of the sucrose metabolic system.
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