Identification of a naturally occurring 2, 6-bis (1.1- dimethylethyl)-4-methylphenol from purple leaves of the halophyte plant Mesembryanthemum crystallinum

Abstract

2, 6-Bis (1.1-dimethylethyl)-4-methylphenol (BHT) is a synthetic antioxidant used generally for food, cosmetics and pharmaceuticals. The leaf extract from the halophyte plant, Mesembryanthemum crystallinum, was fractionated by using semi-preparative HPLC. The different fractions were tested for their antioxidant activity using DPPH method. One fraction exhibited a high level of antioxidant activity. The molecule responsible for this antioxidant activity was identified as 2, 6-bis (1.1-dimethylethyl)-4-methylphenol) by gas chromatography/mass spectroscopy (GC/MS).