Abstract
Surveys were conducted in southwest and west central Florida to identify naturally occurring weed hosts of the whitefly-transmitted tomato mottle geminivirus (TMoV). More than 780 samples representing 42 species in 14 families were collected from 35 field sites over 3 yr. Detection procedures included nucleic acid spot hybridization assays with confirmation by polymerase chain reaction amplification, Southern blot analysis, and virus inclusion visualization. Experimental transmission of TMoV was also attempted to more than 340 plants representing 20 species in seven families by using whiteflies (Bemisia argentifolii [B. tabaci biotype B])
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