Abstract
Eucommia ulmoides Oliv. is a dioecious species. Currently, there is no method to identify the sex during the juvenile stage that lasts a relatively long time. This study aimed to develop a sex specific Amplified Fragment Length Polymorphism (AFLP) marker for Eucommia ulmoides Oliv. Of a total of 64 AFLP primer combinations screened, primer combination E-ACA/M-CTT produced a 350 bp male-specific marker. This 350 bp AFLP marker was converted into a 247 bp Sequence Characterized Amplified Region (SCAR) marker. Results suggest that the SCAR marker can be utilized for early sexual identification in Eucommia ulmoides Oliv., and it will greatly facilitate future breeding programs.
Highlights
Eucommia ulmoides Oliv., called Du-zhong, a dioecious perennial angiosperm, is one of the oldest tonics in Chinese traditional medicine
We have shown that the combined strategy using bulked segregant analysis and Amplified Fragment Length Polymorphism (AFLP) is an efficient method for identifying sex specific markers in Eucommia ulmoides Oliv
We present a male-specific AFLP and Sequence Characterized Amplified Region (SCAR) marker, which was stable in samples from two locations (Henan and Shaanxi province)
Summary
Eucommia ulmoides Oliv., called Du-zhong, a dioecious perennial angiosperm, is one of the oldest tonics in Chinese traditional medicine. The dry stem bark of Eucommia ulmoides Oliv. Was officially listed in the Chinese Pharmacopoeia as an anti-hypertension and bone fracture treatment curative. Recent studies have shown that its bark has many pharmacological actions such as antifungal, Int. J. Du-zhong leaves contain many phyto-chemicals, such as polyphenolics, flavonoids and triterpenoids [6]. Du-zhong tea, the aqueous extract of Eulmoides leaves, has been already known as a functional food and commonly used for reduction of hypertension [7,8]. Gutta-percha is an industrial gum obtained from the seeds, barks and leaves of Eucommia ulmoides Oliv [9,10]
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