Identification of a major quantitative trait locus underlying salt tolerance in \u2018Jidou 12\u2019 soybean cultivar

Xiaolei Shi,Weiwen Yan,David Octor Moseley,Bingqiang Liu,Rui Di,Chunyan Yang,Long Yan,Pengyin Chen,Tao Wang,Mengchen Zhang

doi:10.1186/s13104-018-3202-3

Abstract

BackgroundIdentification of the quantitative trait locus (QTL) underlying salt tolerance is a prerequisite for marker-assisted selection in the salt-tolerant breeding process.MethodsIn this study, the recombinant inbred lines derived from the salt-tolerant elite soybean cultivar ‘Jidou 12’ and the salt-sensitive elite cultivar ‘Ji NF 58’ were used to identify the QTL associated with salt tolerance, using both salt tolerance rating (STR) and leaf chlorophyll content (SPAD) as indicators.ResultsA major salt-tolerant QTL, which was flanked by SSR markers GMABAB and Barcsoyssr_03_1421 on chromosome 3, was identified based on single-marker regression, single trait composite interval mapping, and multiple interval mapping analysis. For STR, the LOD ranged from 19.8 to 20.1; R2 ranged from 44.3 to 44.7%; and the additive effect ranged from 0.876 to 0.885 among the three mapping methods. For SPAD, the LOD ranged from 10.6 to 11.0; R2 ranged from 27.0 to 27.6%; and the additive effect ranged from 1.634 to 1.679 among the three mapping methods.ConclusionsIn this study, a major QTL conditioning salt tolerance on chromosome 3 was identified. The DNA markers closely associated with the QTLs might be useful in marker-assisted selection for soybean salt tolerance improvement in Huanghuaihai, China.

Highlights

Identification of the quantitative trait locus (QTL) underlying salt tolerance is a prerequisite for markerassisted selection in the salt-tolerant breeding process
Because there was a lack of a simple and cost effective way to precisely evaluate a large number of lines for salt tolerance during the selection process in breeding, the development of salt-tolerant soybean cultivars was hampered for a long time
Plant materials A recombinant inbred lines (RIL) population, consisting of 156 lines derived from the cross ‘Jidou 12’ × ‘Ji NF 58’, was used to identify the QTL associated with salt tolerance

Summary

Methods

Plant materials A RIL population, consisting of 156 lines derived from the cross ‘Jidou 12’ × ‘Ji NF 58’, was used to identify the QTL associated with salt tolerance. The linkage map of ‘Jidou 12’ × ‘Ji NF 58’, consisting of 118 SSR markers and covering a total distance of 1008.4 cM on 20 linkage groups, was established. One QTL for both STR and SPAD was detected on chromosome 3, using the averaged data by SMR, CIM, and MIM (Table 1 and Fig. 3). For STR using SMR, this QTL was closely linked with SSR marker Sat_091. For STR using MIM, this QTL was closely linked with SSR marker Sat_091. For SPAD using SMR, this QTL was closely linked with SSR marker Sat_091.

Results

Background

Method