Identification of a Growth-Associated Single Nucleotide Polymorphism (SNP) in Cyclin C of the Giant Tiger Shrimp Penaeus monodon.

Abstract

The full-length cDNA of cyclin C of the giant tiger shrimp Penaeus monodon (PmCyC) was isolated by RACE-PCR. It was 1443bp in length containing an open reading frame (ORF) of 804bp and 267 deduced amino acids. Tissue distribution analysis indicated that PmCyC was more abundantly expressed in ovaries and testes than other tissues of female and male juveniles (P<0.05). A pair of primers was designed, and an amplification product of 403bp containing an intron of 123bp was obtained. Polymorphism of amplified PmCyC gene segments of the 5th (3-month-old G5, N=30) and 7th (5-month-old G7, N=18) generations of domesticated juveniles was analyzed. Four conserved SNPs (T>C134, T>C188, G>A379, and T>C382) were found within the examined sequences. A TaqMan genotyping assay was developed for detection of a T>C134 SNP. Association analysis indicated that this SNP displayed significant association with body weight (P<4.2e-10) and total length (P<2e-09) of the examined G7 P. monodon (N=419) with an allele substitution effect of 5.02±0.78g and 1.41±0.19cm, respectively. Juveniles with C/C134 (22.80±2.51g and 12.97±0.53cm, N=19) and T/C134 (20.41±0.93g and 12.77±0.21cm, N=129) genotypes exhibited a significantly greater average body weight and total length than those with a T/T134 genotype (14.72±0.53g and 11.37±0.13cm, N=271) (P<0.05).