Identification of a fluorescent glucoside isolated from the protein-free extract of human lens

Abstract

One of the fluorescent glucosides associated with yellow pigments was isolated from the protein-free extract of human lens by use of gel filtration and reverse phase HPLC. The isolated compound was identified as 2-amino-3-(β-D-glucopyranosyloxy)-γ-oxobenzenbutanoic acid ( 1) by employing 1H, 13C NMR and mass spectroscopies. The compound was named α-deamino-3-OH-L-Kynurenine-O-β-D-glucoside (DHKN-Glc).