Abstract
The possible expression of FasL in gilthead seabream leucocytes was studied by flow cytometry, immunoblotting and immunocytochemistry, using an anti-mouse FasL monoclonal antibody. The results pointed to a cytosolic FasL-like, but not a membrane-like form, in resting leucocytes from head–kidney, thymus, spleen, blood and peritoneal exudate. Immunoblotting revealed a 19 kDa band in resting leucocytes, while activated leucocytes showed the same band and another of 39 kDa. The FasL-like molecule is identified in lymphocytes, monocyte-macrophages and acidophilic granulocytes. Phylogenetical and functional implications are suggested.
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