Abstract
We have previously demonstrated the stimulation of adenylyl cyclase by guanine nucleotides in the plasma membranes of the aquatic fungus Blastocladiella emersonii. The existence of a G-protein in these membranes similar to Gs, the G-protein responsible for hormonal stimulation of adenylyl cyclase in mammals, was shown. In this report the crude membranes were further analyzed and a polypeptide of ~ 48 kDa reacted with the G α common GA/1 antibody, which recognises the GTP binding site of G-proteins. A polypeptide of similar molecular mass is ADP-ribosylated with pertussis toxin on a cysteine residue, like the counterparts in mammals (Gi, Gt and Go). The appearance of this Gi protein is developmentally controlled, as judged from pertussis toxin labelling and immunoblotting. We suggest the involvement of this G-protein in the control of adenylyl cyclase activity, or ion channels, thus regulating the rapid transition between zoospores and vegetative cells.
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