Abstract
A virus isolated from an infected groundnut seedling grown from seed collected in Jing County, Liaoning Province was identified as a CMV str. (CMV-CA), on the basis of biological, physical and chemical properties, serology and particle morphology. The str. infected 31 of 36 spp. tested (6 families). Longevity in vitro was 6-7 days, thermal inactivation point 55-60°C and dilution end point 10-2-10-3. Macrosiphum euphorbiae transmitted the virus in a nonpersistent manner. Seed transmission in groundnut was c. 1.3%. Virus particles were spherical (28.7 nm diam.). An antiserum against CMV-CA was produced with titres of 1/256 and 1/64 in microprecipitin and gel diffusion tests, respectively. Serologically, CMV-CA was indistinguishable from CMV-D and distinct from CMV-S. There was a more distant serological relationship between CMV-CA and tomato aspermy virus but no serological relationship between CMV-CA and groundnut stunt virus strs. E, W or T. The S20,w value of the particle and mol. wts. of CMV-CA coat protein and of the 4 RNAs agreed with reported values for CMV.
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