Abstract
Within the German national monitoring of zoonotic agents, antimicrobial resistance determination also targets carbapenemase-producing (CP) Escherichia coli by selective isolation from food and livestock. In this monitoring in 2019, the CP E. coli 19-AB01133 was recovered from pork shoulder. The isolate was assigned to the phylogenetic group B1 and exhibited the multi-locus sequence-type ST5869. Molecular investigations, including whole genome sequencing, of 19-AB01133 revealed that the isolate carried the resistance genes blaVIM-1, blaSHV-5 and blaCMY-13 on a self-transmissible IncA/C2 plasmid. The plasmid was closely related to the previously described VIM-1-encoding plasmid S15FP06257_p from E. coli of pork origin in Belgium. Our results indicate an occasional spread of the blaVIM-1 gene in Enterobacteriaceae of the European pig population. Moreover, the blaVIM-1 located on an IncA/C2 plasmid supports the presumption of a new, probably human source of carbapenemase-producing Enterobacteriaceae (CPE) entering the livestock and food chain sector.
Highlights
Carbapenems are last resort antimicrobial agents against several infections caused by multidrug-resistant bacteria in human medicine
The reports of carbapenem resistant bacteria are not limited to human medicine [1]
The corresponding genes are often located on mobile genetic elements, in particular plasmids, which often carry additional antimicrobial, biocide, or heavy metal resistance genes [4]
Summary
Carbapenems are last resort antimicrobial agents against several infections caused by multidrug-resistant bacteria in human medicine. They could persist in the animal host and closely related isolates could be detected in fattening pigs and minced meat in the following years [7,8] Another VIM-1 report described the detection of this gene on an IncY plasmids in E. coli originated from seafood samples in Germany [13]. In 2019 within the annual resistance monitoring in the food chain in Germany, three CP E. coli were isolated These include an OXA-48 producing E. coli [14] and a GES-5 producing E. coli [15], both isolated from pig feces, and another VIM-1 producing E. coli from a pork sample. The genome data hint to a specific human entry source
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