Abstract
Two hitherto unidentified C19 steroids were isolated from adrenal venous blood plasma of female possum ( Trichosurus vulpecula). They were 5α-androstane-3α,17α-diol and its isomer 5β-androstane-3α,17α-diol. The compounds were isolated and identified by fractionation on paper chromatograms and HPLC, followed by gas chromatography-mass spectrometry after conversion to trimethylsilyl ethers. In adrenal venous plasma the concentrations of the 5α-isomer ranged from 24–71 μg/100 ml, 47.5 ± 7.7 (mean ± SEM)s and of the 5β-isomer 1.5–9.3 μg/100 ml, 5.6 ± 1.6 (mean ± SEM). In peripheral plasma only the 5α-derivative was detectable, the highest concentration being 0.97 μg/100 ml. The reduced steroids were not detected in the plasma of a male possum, confirming previous in vitro evidence that reduced steroids are products of the adrenocortical special zone which is found only in the female.
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