Identification of 1BL·1RS Wheat-Rye Chromosome Translocations via 1RS Specific Molecular Markers and Genomic in situ Hybridization

Abstract

Sixty-five rye-specific molecular markers were validated with two 1BL·1RS wheat-rye chromosome translocations(Kavkaz and Shannong 030-1),one 1AL·1RS wheat-rye chromosome translocation(Amigo),Jingzhouheimai,octoploid Triticale Jinsong 49,1R-7R addition lines,Chinese Spring,Huixianhong,Mingxian 169,and Chancellor.Eleven markers were selected due to stable amplification,clear PCR products in electrophoresis gels,and good repeatability,of which eight markers,i.e.,NOR-1,SECA2/SECA3,SCSS30.2,Sec1Gene,Sec1Pro,ω-Sec-P1/P2,ω-Sec-P3/P4,and IB-267 amplified specific bands associated with 1AL·1RS and 1BL·1RS translocations.Another three markers,O-SEC5'-A/O-SEC3'-R,IAG95-1,and SCM-9,were able to discriminate wheat-rye translocations involving different sources of 1RS.Both molecular markers and genomic in situ hybridization were used to detect the frequency of 1BL·1RS translocations in forty Shandong varieties(lines)bred in recent years.Among the forty varieties(lines),only 15%(Weimai 8,Lumai 14,Jining 13,Shannong 664,Shannongyoumai 3,and Yannong 25)harbored the 1BL·1RS translocation with the whole short arm of chromosome 1R of rye,and no 1AL·1RS or other translocation types were found.