Identification of 19-bp indel of the Pleomorphic Adenoma Gene 1 in Bali cattle population

Sukaryo Sukaryo,Joko Riyanto,Muhammad Cahyadi,Rizka Augustin,Yuli Yanti,Slamet Diah Volkandari,Pita Sudrajad,E Widodo,Marjuki Marjuki,S Wahjuningsih,V.M.A Nurgiartiningsih,G Ciptadi,O Sjofjan,Suyadi Suyadi,A Jayanegara,M.H Natsir

doi:10.1051/e3sconf/202233500011

Abstract

Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor gene located on bovine chromosome 14 (BTA14) affecting body size and reproduction traits in cattle. The objective of this study was to identify 19-bp indel of the PLAG1 gene in Bali cattle population. A total of 96 blood samples of Bali Cattle were collected from Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU-HPT) Denpasar. Genomic DNA was extracted from blood samples and used to detect 19-bp indel of the PLAG1 gene using following primer pair 5’-TCCGAACAACAGGTGAGGGAGAAAT-3’ and 5’-CCACTTCAGG-GGTGCTCTAGGTTTG-3’. The polymerase chain reaction (PCR) products using DNA pool samples were sequenced to validate the PCR product and to find out novel polymorphism in Bali cattle population. The result showed that there was no variation found in Bali cattle population based on 19-bp indel of the PLAG1 gene, which is indicated by 123 bp DNA band. However, sequence analysis of the PLAG1 gene resulted in a novel single nucleotide polymorphism (SNP) at nucleotide number 32235 of the PLAG1 gene that changed guanine (G) to adenine (A). This novel SNP could be furthermore genotyped and it might be a potential candidate marker for body size and reproduction traits in Bali cattle.

Highlights

Cattle is one of livestock commodity producing meat who are considerable contributing to national beef supply
Amplification of the Pleomorphic adenoma gene 1 (PLAG1) gene fragment in Bali cattle population resulted 123 bp polymerase chain reaction (PCR) product (Figure 1). This result was in line with those predicted for detection 19-bp indel of the PLAG1 gene
It showed that the DNA isolated from Bali cattle blood was adequate to be used for PCR

Summary

Introduction

Cattle is one of livestock commodity producing meat who are considerable contributing to national beef supply. It is very important to improve productivity and population of Indonesian local cattle, especially whose genetically superior such as Bali cattle (Bos javanicus). Body weight and measurement traits are the easiest growth traits to be observed consisting of birth weight, actual body weight, withers height, body length, chest girth, waist height, and chest depth [3]. Those traits are well-known as quantitative traits which are economically and biologically important traits and able to be used as indicators in selecting superior Bali cattle [4]. The development of recent molecular technique allows human being to improve native cattle production genetically through marker assisted selection

Objectives

Methods

Results