Abstract
SUMOylation is a kind of important post-translational modification. In the present study, we identified 10 key genes involved in SUMOylation and deSUMOylation (sumo1, sumo2, sumo3, sae1, uba2, ubc9, pias1, senp1, senp2, and senp3) in yellow catfish Pelteobagrus fulvidraco, investigated their tissue expression patterns and transcriptional responses to carbohydrate addition both in vivo and in vitro. All of these members shared similar domains to their orthologous genes of other vertebrates. Their mRNAs were widely expressed in all the tested tissues, but at variable levels. Dietary carbohydrate levels differentially influenced the mRNA levels of these genes in liver, muscle, testis, and ovary of yellow catfish. Their mRNA levels in primary hepatocytes were differentially responsive to glucose addition. Our study would contribute to our understanding into the molecular basis of SUMOylation modification and into the potential SUMOylation function in the carbohydrate utilization in fish.
Highlights
SUMOylation is a kind of important post-translational modification
The present study identified the cDNA sequences of 10 key genes in small ubiquitin-related modifier (SUMO)- and deSUMOylation in yellow catfish, and examine their transcriptional responses in several tissues of yellow catfish fed different levels of dietary carbohydrate
The amino acid identities of P. fulvidraco sumo1, sumo2, sumo3, sae1, uba2, ubc9, pias1, senp1, senp2, and senp3 were similar to those of orthologous from other species, exhibiting 28.12–100% amino acid sequence identities among different species
Summary
SUMOylation is a kind of important post-translational modification. It is characterized by SUMO proteins which covalently binds to the ε-amino group of lysine residues in intracellular target proteins in an ATP-dependent process (Gareau and Lima, 2010). During the SUMOylation modification, a series of enzymatic reactions mediate the process. These enzymes include the heteromeric activating enzyme E1 (Uba2), the conjugation enzyme E2 (Ubc9), and SUMO E3 ligases represented by PIAS family (Johnson, 2004; Hay, 2005). SUMOylation is a dynamic process which can be reversed by a family of Sentrin/SUMO-specific protease (Bailey and O’Hare, 2004; Hay, 2005). SENPs act to deSUMOylate the proteins by cleaving the covalent conjugation between SUMO and its target (Wilkinson and Henley, 2010)
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