Abstract
In the present study, IL-1β cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length IL-1β mRNA was obtained using Rapid Amplification of cDNA Ends (RACE), which contains 78 bp 3′-UTR, a 455 bp 5′-UTR, and an open reading frame (ORF) of 702 bp coding for 233 amino acid residues. The molecular weight and theoretical isoelectric point of largemouth bass IL-1β protein was predicted to be 26.7 kDa and 6.08 respectively. A largemouth bass IL-1β phylogenetic analysis showed a close relation to the IL-1βs of striped trumpeter (Latris lineata), Chinese perch (Siniperca chuatsi), and Japanese sea bass (Lateolabrax japonicus). Peptidoglycan upregulated IL-1β in the spleen and head kidney, while lipopolysaccharide upregulated detectable levels of IL-1β in the spleen only. Largemouth bass, challenged with Nocardia seriolae (1.0 × 106 cfu/mL), showed a significant increase in IL-1β at 3 and 5 days post infection (dpi) in the spleen, while in the head kidney significant expression was found at 2 and 3 dpi, peaking at 3 dpi. Furthermore, tumor necrosis factor α (TNF-α) showed significantly higher expression in the spleen at 3 and 5 dpi, and in the head kidney at 1 and 3 dpi, with expression decreasing at 5 dpi in both tissues.
Highlights
Interleukin-1β is a member of the interleukin-1 family of cytokines that mediates the response of the host to microbial invasion, inflammation and immune reactions
Nocardia seriolae is the etiological agent of fish nocardiosis [19], which is characterized by the formation of abscesses on the epidermis and granulomas in the gills, kidney, and spleen, and is a serious disease for cultured yellowtail Seriola quinqueradiata and amberjack S. aureovittata [20]
We evaluated an important component tumor necrosis factor α (TNF-α), which can be secreted by different cell types through the stimulation with inflammatory mediators, or cytokines such as IL-1β
Summary
Interleukin-1β is a member of the interleukin-1 family of cytokines that mediates the response of the host to microbial invasion, inflammation and immune reactions. Nocardia seriolae is the etiological agent of fish nocardiosis [19], which is characterized by the formation of abscesses on the epidermis and granulomas in the gills, kidney, and spleen, and is a serious disease for cultured yellowtail Seriola quinqueradiata and amberjack S. aureovittata [20]. It had been diagnosed in disease outbreaks in a range of fish species, including freshwater [21,22,23,24] and seawater fish [25,26,27]. We evaluated an important component tumor necrosis factor α (TNF-α), which can be secreted by different cell types through the stimulation with inflammatory mediators, or cytokines such as IL-1β
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