Identification methods of Candida isolated from vaginal infections

Aya Mousa,Marwa Azab,Ali Abdelrahman

doi:10.21608/rpbs.2019.9792.1021

Abstract

A number of methods of sampling the vagina for the presence of candida have been developed. Such techniques play an important role in the diagnosis and management of vaginal candidiasis. In the past, identification of candida isolated from the vagina has usually been limited to the genus Candida or to the species C. albicans. However, with the recognition that Candida species differ in the production of putative virulence factors and sensitivity to antifungal agents, greater emphasis has been placed on identification of isolates to species level. As a result, a range of commercially available systems for yeast identification can now be used in conjunction with traditional identification procedures. In this work, Candida detected by many phenotypic identification methods such as sabaroud dextrose agar medium, microscopical identification, sugar fermentation test and germ tube test. Polymerase chain reaction (PCR) also used as a fast and sensitive genotypic technic to identify Candida sp.

Highlights

Candida is as an opportunistic pathogen, presenting at the oral cavity, the gastrointestinal tract, the vaginal and the urinary environments (Pfaller and Diekema 2007)
The present study was conducted with a total of 157 women clinically diagnosed of genitourinary tract infections and by using the conventional culture technique only 100 isolates were identified as Candida with a prevalence rate (64%)
3.2.3 Sugar fermentation test By these test 91 samples identified as Candida albicans and 9 samples identified as Candida glabrata

Summary

Introduction

Candida is as an opportunistic pathogen, presenting at the oral cavity, the gastrointestinal tract, the vaginal and the urinary environments (Pfaller and Diekema 2007). C. albicans commonly causes infections such as denture stomatitis, thrush, and urinary tract-infections, and can make more severe systemic infections. These are frequently life-threatening, in immunocompromised individuals, whose numbers are constantly increasing due to organ transplant, chemotherapy, or, more importantly, to the prevalence of AIDS and Hepatitis C (Pfaller and Diekema 2007). Many microbiological laboratories providing a mycology service limit the level of yeast identification to Candida species only and the demonstration of antifungal susceptibility (Odds and Bernaerts, 1994). It is becoming recognised that isolates of Candida species differ widely, both in their ability to cause infection (Cázares-Núñez et al, 2017; Odds and Bernaerts, 1994) and alsoin their susceptibility to antifungal agents (McCullough et al, 2004). This review provides an overview for the techniques currently available for identification of isolates

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Conclusion