Abstract
Abscisic acid (ABA) plays a crucial role in various aspects of plant growth and development, including fruit development and ripening, seed dormancy, and involvement in response to various environmental stresses. In almost all higher plants, ABA signal transduction requires three core components; namely, PYR/PYL/RCAR ABA receptors (PYLs), type 2C protein phosphatases (PP2Cs), and class III SNF-1-related protein kinase 2 (SnRK2s). The exploration of these three core components is not comprehensive in soybean. This study identified the GmPYL-PP2C-SnRK2s gene family members by using the JGI Phytozome and NCBI database. The gene family composition, conservation, gene structure, evolutionary relationship, cis-acting elements of promoter regions, and its coding protein domains were analyzed. In the entire genome of the soybean, there are 21 PYLs, 36 PP2Cs, and 21 SnRK2s genes; further, by phylogenetic and conservation analysis, 21 PYLs genes are classified into 3 groups, 36 PP2Cs genes are classified into seven groups, and 21 SnRK2s genes are classified into 3 groups. The conserved motifs and domain analysis showed that all the GmPYLs gene family members contain START-like domains, the GmPP2Cs gene family contains PP2Cc domains, and the GmSnRK2s gene family contains S_TK domains, respectively. Furthermore, based on the high-throughput transcriptome sequencing data, the results showed differences in the expression patterns of GmPYL-PP2C-SnRK2s gene families in different tissue parts of the same variety, and the same tissue part of different varieties. Our study provides a basis for further elucidation of the identification of GmPYL-PP2C-SnRK2s gene family members and analysis of their evolution and expression patterns, which helps to understand the molecular mechanism of soybean response to abiotic stress. In addition, this provides a conceptual basis for future studies of the soybean ABA core signal pathway.
Highlights
The results show that GmPYL-PP2C-SNF1-Related protein kinases2 (SnRK2) gene families have a wide range of
The GmPYL-PP2C-SnRK2s genes are involved in plant abiotic stress responses, which has been documented in many species, as the core genes of the Abscisic acid (ABA) core signaling pathway
Gene structure, conserved sequence, and domain analysis have shown that members of the GmPYL-PP2C-SnRK2 gene families are evolutionarily relatively conservative
Summary
Merr) is an essential leguminous crop that provides the human diet with an essential protein source, livestock feed, and industry biodiesel [1]. The sustainability of soybean yields is challenged by expected changes in the climate with prolonged drought, salinity, and high temperature in many areas of the world [2]. Many innate survival mechanisms have evolved to respond to these adverse conditions, a crucial metabolic process called abscisic acid accumulation [3]. Abscisic acid (ABA) is a key regulator for plant stress-adaptation; the ABA signaling pathways are made up of many important turnover mechanisms in response to ever-changing environmental conditions [4]. The PYL-PP2C-SnRK2s family of proteins are the main members of the ABA core signaling pathway
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
