Abstract
BackgroundMitochondrial transcription termination factor (mTERF) is a large gene family which plays a significant role during plant growth under various environmental stresses. However, knowledge of mTERF genes in grapevine (Vitis L.) is limited.ResultsIn this research, a comprehensive analysis of grape mTERF (VvmTERF) genes, including chromosome locations, phylogeny, protein motifs, gene structures, gene duplications, synteny analysis and expression profiles, was conducted. As a result, a total of 25 mTERF genes were identified from the grape genome, which are distributed on 13 chromosomes with diverse densities and segmental duplication events. The grape mTERF gene family is classified into nine clades based on phylogenetic analysis and structural characteristics. These VvmTERF genes showed differential expression patterns in response to multiple phytohormone treatments and biotic stresses, including treatments with abscisic acid and methyl jasmonate, and inoculation of Plasmopara viticola and Erysiphe necator.ConclusionsThese research findings, as the first of its kind in grapevine, will provide useful information for future development of new stress tolerant grape cultivars through genetic manipulation of VvmTERF genes.
Highlights
Mitochondrial transcription termination factor is a large gene family which plays a significant role during plant growth under various environmental stresses
Phylogenetic analysis and classification of grape mitochondrial transcription termination factors (mTERFs) genes In order to evaluate the evolutionary relationship of VvmTERF gene family, a total of 91 mTERF genes from Arabidopsis (35), maize (31) and grape (25) genomes were collected for a phylogenetic tree construction using MEGA5.0 software (Fig. 1 and Figure S1)
Significant progresses have been made in identifying mTERF genes in model plant species, little information of mTERF genes has been known in fruit crops
Summary
Mitochondrial transcription termination factor (mTERF) is a large gene family which plays a significant role during plant growth under various environmental stresses. Genetic information is stored in the nucleus, and in organelle genomes such as mitochondria and chloroplasts. Mitochondrial transcription termination factor (mTERF) genes comprise a large family which plays an essential role in the regulation of mitochondrial gene. Human mTERF1, with 342 amino acids in length, can bind to 28 nucleotide sequences downstream of the 3’end of 16SrRNA, leading to the termination of mitochondrial gene transcription [13, 14]. The mTERF1 protein possess regulatory function for transcriptional initiation of mitochondrial rDNA and mitochondrial DNA replication [15]. MTERF2 protein showed a significant downregulation of mitochondrial transcription level in vitro, suggesting that mTERF2 protein may affect mitochondrial transcription by binding with regulatory activators of mtDNA transcription initiation [16]
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