Abstract

In sections of the pituitary of the newt treated with fluorescent antiprolactin globulin, a green fluorescent antigen-antibody complex is present only in the acidophilic cells localized in the dorsocentral region of the distal lobe. These cells are identifiable with type I acidophils according to Mazzi (1949) and with type II acidophils according to van Oordt (1968). These results are in keeping with the observation that purified newt prolactin obtained by polyacrylamide disc electrophoresis reacts immunologically with rabbit antisheep prolactin antiserum.

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