Abstract
Hypericum perforatum is a medicinal plant which has been known in traditional medicine as an anti-inflammatory and healing agent. Nowadays, the alcoholic extract of its aerial parts finds wide application for its antidepressant activity. A high-performance liquid chromatography (HPLC) method for the identification of its constituents using a wide pore RP-18 column and a water–methanol–acetonitrile–phosphoric acid mobile phase system was developed. The identification of its flavonoid, naphthodianthrone and phloroglucinol constituents was performed using combined HPLC–diode array detection (DAD) analysis, HPLC–thermospray and HPLC–electrospray mass spectrometry. Chlorogenic acid, quercetin, quercitrin, isoquercitrin, rutin, hyperoside, I3,II8-biapigenin, pseudohypericin, hypericin, hyperforin and adhyperforin were separated by an aqueous phosphoric acid–acetonitrile–methanol gradient within 50 min. The quantification of the above constituents was performed using rutin as an external standard.
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