Abstract
BackgroundIntratumoural CD103+CD8+ T cells have been linked to prolonged survival in several malignancies. However, the clinical significance of CD103+CD8+ T cells in gastric cancer remains unexplored.MethodsGastric cancer tissues from Zhongshan Hospital and data from Gene Expression Omnibus were obtained and analysed. Immunohistochemistry and flow cytometry were performed to detect the number and phenotypical characteristics of CD103+CD8+ T cells. The effect of programmed cell death protein-1 (PD-1) blockade on CD103+CD8+ T cells was evaluated with the use of an in vitro study based on fresh tumour tissues.ResultsCD103+CD8+ T cells predicted superior overall survival and provided better prognostic power than total CD8+ T cells in gastric cancer. Patients with high CD103+CD8+ T cell infiltration also gained more benefit from adjuvant chemotherapy. Flow cytometry analysis showed that CD103+CD8+ T cells exerted superior anti-tumour effects with stronger retention capacity and cytotoxicity. Moreover, an in vitro study showed that CD103+CD8+ T cells were more functionally restored after PD-1 blockade than CD103-CD8+ T cells.ConclusionsCD103+CD8+ T cells might be a useful marker to predict prognosis and therapeutic efficacy for gastric cancer patients. Efforts to increase intratumoural CD103+CD8+ T cell frequency might be a novel therapeutic strategy in gastric cancer.
Highlights
Intratumoural CD103+CD8+ T cells have been linked to prolonged survival in several malignancies
CD103+CD8+ T cells compared with total CD8+ T cells, we further explored the phenotypic characteristics of intratumoural
We found that intratumoural CD103+CD8+ T cells in gastric cancer exhibited a HOBIThigh/BLIMP-1low phenotype, which was inconsistent with findings in human lung tissues.[38]
Summary
Intratumoural CD103+CD8+ T cells have been linked to prolonged survival in several malignancies. Gastric cancer is the fifth most common cancer and the third leading cause of cancer-related death worldwide.[1] In recent years, significant progress has been made in the prevention, diagnosis and therapeutic strategies of gastric cancer, many questions remain unanswered, especially the prediction of prognosis and therapeutic response in gastric cancer.[2] Currently, it is generally believed that the pathogenies and progression of gastric cancer are influenced by the cross-talk between tumour cells and the host immune system.[3,4,5] the prognostic and predictive value of tumour-infiltrating immune cells in gastric cancer has drawn more attention in the past ten years.[6,7,8].
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