Abstract
BackgroundThe red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. The identification of odorant binding protein (OBP) genes will be helpful for clarifing the mechanism of odorant detection of this pest. By sequencing the full length cDNA library of its antenne, 11 OBP genes (RferOBP1-11) were identified.FindingsThe result showed RferOBP1-7 and RferOBP8-11 belonged to the minus-C and classic family, respectively qPCR revealed that RferOBP1-10 highly transcribed in the antennae, of which RferOBP1, RferOBP4, RferOBP8 and RferOBP10 were obviously male-biased expression. RferOBP7 and RferOBP11 exhibited highly expression in female head and male thorax. RferOBP2, RferOBP5 and RferOBP6 were highly expressed in the female thorax, leg and abdomen respectively.ConclusionsThe results paved the way towards a future understanding of the olfaction in this species.
Highlights
The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms
Characteristics of odorant binding protein (OBP) sequences In total, 11 full length OBP genes from 36 clones named as RferOBP1-11 were identified from the antennal cDNA library sequencing, which have been deposited in the NCBI under the accession numbers KR780571 to KR780581 (Table 2)
Compared to the 49 OBPs identified in the genome of Tribolium castaneum, more OBP genes might be expected to be identified in R. ferrugineus by massive sequencing strategies and by using more different tissues that may express OBPs (Tribolium Genome Sequencing Consortium 2008; Zhu et al 2013)
Summary
The red palm weevil, Rhynchophorus ferrugineus, is a lethal pest of the palms. Findings: The result showed RferOBP1-7 and RferOBP8-11 belonged to the minus-C and classic family, respectively qPCR revealed that RferOBP1-10 highly transcribed in the antennae, of which RferOBP1, RferOBP4, RferOBP8 and RferOBP10 were obviously male-biased expression. RferOBP7 and RferOBP11 exhibited highly expression in female head and male thorax. RferOBP2, RferOBP5 and RferOBP6 were highly expressed in the female thorax, leg and abdomen respectively. The red palm weevil, Rhynchophorus ferrugineus, belongs to Coleoptera: Dryophthoridae family and is native to tropical Asian regions which have become a most threatening pest of palm trees worldwide (Yan et al 2015). Due to its concealed nature and exceptional colonization capability, integrated pest management strategies play an important role in controlling the red palm weevil (Vacas et al 2014).
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