Abstract
To identify biomarkers of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234)- or methyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A232)-inhibited butyrylcholinesterase (BChE), we investigated nonapeptide adducts containing the active site serine, which plays a key role in enzyme activity, using LC-MS/HRMS. Biomarkers were acquired as expected, and they exhibited a significant amount of fragment ions from the inhibiting agent itself, in contrast to the MS2 spectra of conventional nerve agents. These biomarkers had a higher abundance of [M+2H]2+ ions than [M+H]+ ions, making doubly charged ions more suitable for trace analysis.
Highlights
Novichok is a new type of organophosphorus nerve agent (OPNA) and is highly toxic because it rapidly inactivates cholinesterase (ChE) activity [1]
[16,17], and many verification studies support its reactivity against agent of human acetylcholinesterase [15] to block the degradation of acetylcholine, causing these studies, we postulated thatofNovichok agents inhibit the failureFrom of the nervous system
We believe that the structure of nerve agents might play a role, compared to the data from VX and GB, so we are going to find a relation between the structure and ion ratio by researching with other OPNAs
Summary
Novichok is a new type of organophosphorus nerve agent (OPNA) and is highly toxic because it rapidly inactivates cholinesterase (ChE) activity [1]. Its identity was confirmed by the Organization for the Prohibition of Chemical Weapons (OPCW) [3]. After this poisoning attack, Novichok was newly included in the Chemical. [4] The intention to use of these warfare agents was further found in the poisoning case of Alexei Navalny in 2020. These agents were initially confirmed by Vil Mirzayanov, and are known as chemical agents developed under the FOLIANT project of the USSR [5,6]. Despite the recent discovery and the legal restrictions on the acquisition of these compounds, a series of studies have been conducted on their toxicity and physical properties [6,7], simulated toxicity in silico [8], degradation conditions, and corresponding pathways [9,10,11]
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