Abstract
Malolactic fermentation conducted by Leuconostoc œnos is an essential step in winemaking. L. œnos bacteriophages are thought to be responsible for fermentation failures, yet they have received little attention. The integration system of bacteriophage φ10MC in the LOF111 L. œnos strain chromosome was studied and a 1456 bp phage DNA fragment was cloned and sequenced. An open reading frame ( int) showing homology with several temperate bacteriophage integrases was located upstream of the phage attachment site ( attP). This organisation is comparable to other phage site-specific recombination systems. The same bacterial attachment site ( attB) located in a tRNA leu gene was found in all 15 L. œnos strains studied and was involved in φ10MC integration in the bacterial chromosome.
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