Abstract
In the present study, we utilized high throughput and Sanger sequencing to determine the complete nucleotide sequence of a putative new ilarvirus species infecting sweet cherry, tentatively named prunus virus I (PrVI). The genome of PrVI is comprised of three RNA segments of 3474 nt (RNA1), 2911 nt (RNA2), and 2231 nt (RNA3) and features conserved motifs representative of the genus Ilarvirus. BlastN analysis revealed 68.1–71.9% nt identity of PrVI with strawberry necrotic shock virus (SNSV). In subsequent phylogenetic analysis, PrVI was grouped together with SNSV and blackberry chlorotic ringspot virus (BCRV), both members of subgroup 1 of ilarviruses. In addition, mini-scale surveys in stone fruit orchards revealed the presence of PrVI in a limited number of sweet cherries and in one peach tree. Overall, our data suggest that PrVI is a novel species of the genus Ilarvirus and it consists the fifth member of the genus that is currently known to infect Prunus spp.
Highlights
Prunus spp. are infected by a significant number of plant viruses, including at least four members of the genus Ilarvirus: apple mosaic virus (ApMV), American plum line pattern virus (APLPV), prune dwarf virus (PDV), and prunus necrotic ringspot virus (PNRSV)
RNA-1 and RNA-2 encode proteins associated with replication, whereas RNA-3 harbors open reading frames (ORFs) that encode the movement (MP) (5 terminal) and the coat protein (CP) (3 terminal)
The complete genome sequence of prunus virus I (PrVI) was confirmed by Sanger sequencing and Rapid amplification of cDNA ends (RACE)
Summary
Prunus spp. are infected by a significant number of plant viruses, including at least four members of the genus Ilarvirus (family Bromoviridae): apple mosaic virus (ApMV), American plum line pattern virus (APLPV), prune dwarf virus (PDV), and prunus necrotic ringspot virus (PNRSV). Among these viruses, PDV and PNRSV are the most prevalent and have been associated with various stone fruit diseases, such as “ringspot disease” or “peach stunt disease” [1]. Ilarviruses have four to five open reading frames (ORFs) encoded by their tripartite, positive-sense, singlestranded RNA genome. Based on serological and molecular data, ilarviruses are clustered into four subgroups, of which subgroups 1 and 2 encode an additional protein, named 2b (3 terminal of RNA-2), putatively associated with silencing suppression activity [2,3]
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