Abstract
BackgroundAmong the huge diversity of thermophilic bacteria mainly bacilli have been reported as active thermostable lipase producers. Geothermal springs serve as the main source for isolation of thermostable lipase producing bacilli. Thermostable lipolytic enzymes, functioning in the harsh conditions, have promising applications in processing of organic chemicals, detergent formulation, synthesis of biosurfactants, pharmaceutical processing etc.ResultsIn order to study the distribution of lipase-producing thermophilic bacilli and their specific lipase protein primary structures, three lipase producers from different genera were isolated from mesothermal (27.5–70 °C) springs distributed on the territory of Armenia and Nagorno Karabakh. Based on phenotypic characteristics and 16S rRNA gene sequencing the isolates were identified as Geobacillus sp., Bacillus licheniformis and Anoxibacillus flavithermus strains. The lipase genes of isolates were sequenced by using initially designed primer sets. Multiple alignments generated from primary structures of the lipase proteins and annotated lipase protein sequences, conserved regions analysis and amino acid composition have illustrated the similarity (98–99%) of the lipases with true lipases (family I) and GDSL esterase family (family II). A conserved sequence block that determines the thermostability has been identified in the multiple alignments of the lipase proteins.ConclusionsThe results are spreading light on the lipase producing bacilli distribution in geothermal springs in Armenia and Nagorno Karabakh. Newly isolated bacilli strains could be prospective source for thermostable lipases and their genes.
Highlights
Among the huge diversity of thermophilic bacteria mainly bacilli have been reported as active thermostable lipase producers
The present study focuses on isolation and identification lipase-producing thermophilic bacilli from geothermal springs of Armenia (Akhourik and Tatev) and NagornoKarabakh (Karvachar) as well as comparative analysis of their lipase encoding genes’ sequences
In total forty-one chemoorganotrophic thermophilic aerobic lipase-producer bacilli strains were isolated from Akhourik, Tatev and Karvachar geothermal springs
Summary
Among the huge diversity of thermophilic bacteria mainly bacilli have been reported as active thermostable lipase producers. Geothermal springs serve as the main source for isolation of thermostable lipase producing bacilli. Thermophilic microorganisms are gaining big industrial and biotechnological interest due to their enzymes (thermozymes) remaining active in harsh industrial processes [1]. The industrial demand for the thermozymes continues to stimulate the search for novel thermophilic microorganisms from various unexplored regions of the Earth, such as deep-sea hydrothermal vents, terrestrial geothermal springs and mudpots [2]. One of the important groups of biotechnologically relevant enzymes are lipases (EC 3.1.1.3 - triacylglycerol hydrolases), which have found large applications in food, diary, detergent, and pharmaceutical industries [3, 4].
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