Abstract
Identification and screening of Citrus Vein Phloem Degeneration (CVPD) on Brastagi citrus variety Brastepu (Citrus nobilis Brastepu) in North Sumatra Indonesia is explained. The aim of the study is to explore the technique to obtain healthy Brastepu citrus to be used as a source of explants for in vitro propagation as a step in the preservation of threatened local citrus. The studies were conducted through collection of survived Brastepu citrus followed by screening the CVPD in the plants. Various procedures in the field and in the laboratory have been conducted to screen infected CVPD in Brastepu citrus. Visual examination from leaf, fruit, and seed appearance becomes preliminary information for CVPD infection in the citrus plant. Iodine test was used to support results obtained by visual observation. Histochemical test and the PCR analysis have confirmed that 12 samples of Brastepu citrus are free from CVPD and eight citrus are infected by CVPD. Adequate informations have been obtained to confirm healthy citrus tree which are then to be used as sources of explants for in vitro propagation in the preservation of Citrus nobilis Brastepu.
Highlights
Brastagi citrus to be known as “Jeruk Brastagi” is very popular local sweet orange many years ago and become one of the well known commodities from Brastagi, North Sumatra, Indonesia
Citrus nobilis Brastepu which was called as Brastepu citrus has superior potential genetic compared with honey citrus and other local citrus, where the fruit is large in size, has sweet taste, and a raped peel is yellow to reddish color
The aim of the research is to explain a rapid screening technique to determine the presence of infected Citrus Vein Phloem Degeneration (CVPD) in Citrus nobilis Brastepu, a threat species of Brastagi citrus in North Sumatra Indonesia, in order to obtain healthy plants to be used as sources of explants for in vitro propagation of Brastepu citrus as a step in the preservation of threatened local citrus
Summary
Brastagi citrus to be known as “Jeruk Brastagi” is very popular local sweet orange many years ago and become one of the well known commodities from Brastagi, North Sumatra, Indonesia. Brastagi citrus nowadays are grown in the field as unintended plants because they are not planted properly with some reasons, the production quantity and their sensitivity to some diseases such as CVPD. It has been informed by citrus farmer that the CVPD has destroied Brastagi citrus and nowadays it has been replaced by imported citrus. It is need to screen healthy survived trees to be used as sources of explants for in vitro propagation in the conservation of Brastepu citrus. The use of plants pathogen free is believed to be an efficient strategy to control citrus disease, and this strategy is chosen to obtain healthy citrus trees with free from CVPD which is to be used as explants sources in the propagation of Brastepu citrus
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