Abstract
Vegetable crops are inexhaustible source of biologically active compounds. Cabbage (Brassica oleracea L.) – is a plant that has been extensively used in folk medicine of different countries of the world for the treatment of various disorders, and has anti-inflammatory, expectorant, broncholytic, diuretic, and genera tonic effect. The plant material of cabbage in not officinal in Ukraine, thus it requires a complex pharmacognostic research to be carried out. The aim of the research. Identification and quantitative determination of steroidal compounds in the leaves, seeds and heads of cabbage. Materials and methods. Identification of steroidal compounds and determination of their quantity in the plant material of cabbage was carried out using gas chromatography/mass-spectrometry (GC/MS) method. The results of the study and their discussion . As a result of the carried out experiment 3 compounds of steroidal nature were identified in the leaves of cabbage of “Snow-white” variety, and 4 – of “Ukrainian autumn” and “Yaroslavna” varieties, 4 compounds in the seeds of cabbage of “Snow-white” variety, 3 – of “Ukrainian autumn” and “Yaroslavna” varieties, and 3 steroidal compounds in the heads of cabbage of all the varieties. β-Sitosterol was the dominating compound by the content in all studied samples. Its highest content was found in the leaves (2499 mg/kg) and seeds (1728 mg/kg) of cabbage of “Yaroslavna” variety, as well as in the heads (1148 mg/kg) of cabbage of “Ukrainian autumn” variety. Conclusion . Results of the experiment can be used at the quality control methods for the cabbage plant material development as well as obtaining prospective biologically active substances from the studied plant material
Highlights
The study of crops as sources of medicinal preparations obtaining on their basis is actual for pharmacy nowadays
Formulation of article’s tasks For the purpose of in-depth study the qualitative composition and quantitative content of steroidal compounds was determined by the gas chromatography / mass spectrometry (GC/MS) method in leaves, seeds and stumps of cabbage of "Snow White", "Ukrainian Autumn" and "Yaroslavna" varieties as they are in
It should be noted that the total quantity of steroids in the raw material of cabbage of "Snow White" variety dominated in seeds (784.00 mg/kg) and stumps (309,00 mg/kg), for "Ukrainian autumn" varieties in the leaves (1430.00 mg/kg) and stumps (1672.00 mg/kg), for "Yaroslavna"variety – in the leaves (4035.00 mg/kg) and seeds (3041.00 mg/kg)
Summary
The study of crops as sources of medicinal preparations obtaining on their basis is actual for pharmacy nowadays. The leaves, roots, stumps, and seeds of the plant are use [1]. It is known that anti-inflammatory activity is exhibited by such a class of biologically active compounds as steroids. According to the literature data, in the medicine of different nations the raw materials of plants of the cabbage family are used to treat diseases that are accompanied by inflammatory processes [10, 11]. The study of steroidal compounds in leaves, seeds and stumps of cabbage can be expedient for their comprehensive study. 3. Analysis of recent research and publications The analysis of literary sources showed that the cabbage reveals multi-vector pharmacological activity, in particular anti-inflammatory, antibacterial, expectorant, bronchodilatory, diuretic, general strengthening activity [11]. Their study in the leaves, seeds and stumps of cabbage of the investigated varieties is of current interest
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