Abstract
The 31P NMR-spectroscopy-based spin trap technique involving 5-diisopropoxy-phosphoryl-5-methyl-1-pyrroline-N-oxide (DIPPMPO) was used to achieve quantitative analyses of the radical species that are generated in different bleaching solutions. These solutions comprised a mixture of an ammonium salt and hydrogen peroxide. This spin trap-based approach was also applied, with modifications, to the study of bleaching systems of human hair. The obtained results clearly revealed the subtle differences in both the nature and the amounts of the radical species generated in different bleaching solutions, and when allowed to react on hair samples. Generally, the main species involved in the oxidation processes were superoxide and amino radicals. Their amounts, however, showed a significant variation upon the kind of bleaching system and nature of the hair, i.e., virgin or dyed hair.
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