Identification and quantification of phenolic compounds through reversed phase HPLC-DAD method in sunflower seeds under various treatments of potassium nitrate, zinc sulphate and gibberellic acid

Abstract

Phenolic compounds were extracted from seeds of eight varieties i.e. Rising Sun, SMH-0907, Ausigold-7, SMH-0939, US-444, Hysun-33, SMH-0917 and HS-K6 of sunflower and characterized by HPLC-DAD method. Quantification of individual compounds was carried out by external calibration. Total of 15 phenolic compound were identified, namely hydroxybenzoic acid, hydroxybenzoyl glucose, caftaric acid, rosmanol, gallic acid derivative, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, caffeic acid, pro-anthocynidin B1, rosmannic acid, sinapic acid derivative, caffeic acid derivative 1, caffeic acid derivative 2, caffeoylmalic acid derivative and quercetin derivative in the seeds of sunflower. Different treatments of potassium nitrate, zinc sulphate, and gibberellic acid showed significant effect on the biosynthesis of phenolic compound as compared to control plants. Among the 15 compounds analyzed 3,4-di-O-caffeoylquinic acid was predominant compound in the seeds. The current results demonstrate that this method can be efficiently used for the identification and quantification of phenolic compound in sunflower seeds.