Identification and profiling of silicate-solubilizing bacteria for plant growth-promoting traits and rhizosphere competence

Abstract

Excessive use of synthetic fertilizers may negatively affect overall soil health and the environment. There is a need to come up with alternative strategies to ensure competitive yields of crops and at the same time maintain soil ecological balance. The use of soil microorganisms as microbial inoculants in agriculture is considered an alternative approach to enhance crop growth. Soil is a good source of microorganisms that are capable of producing different beneficial enzymes such as silicase, phosphatase and other growth-promoting enzymes which are important in crop growth. Soil beneficial microorganisms such as silicate-solubilizing bacteria play an important role in solubilizing insoluble forms of silicates that are important in crop growth. This study was conducted to identify and profile silicate-solubilizing bacteria (SSB) in Louisiana soils and to document the colonization potential of SSB in rice (Oryza sativa) plants. Bacterial isolates were spot-inoculated on silica agar medium and incubated at 37 °C for 48 h. The clearing zone around colonies was measured and used to compute for solubilization index (SI), i.e., halo diameter (mm)/colony diameter (mm). Despite of the abundance of microorganisms found in these soils only 20 bacterial isolates are capable of solubilizing silicates; 16 were with low (SI < 2.0) and 4 with intermediate (2.00 < SI < 4.00) solubilization capacities. Further analysis also revealed that three of the isolates with intermediate silicate solubilization capacities also produced plant growth-promoting enzymes: phosphatase, 1-aminocyclopropane-1-carboxylic acid deaminase and indole-3-acetic acid enzyme. Based on the sequencing information and the phylogenetic tree, the putative SSB were identified into four genera: Aeromonas, Bacillus, Enterobacter and Pseudomonas. The fluorescent microscopy analysis revealed that the GFP-tagged SSB can colonize roots of young rice seedlings, a good indicator of SSB potential as seed treatment for scaling up its application in production field. Furthermore, information on the survival of SSB inoculates in different soil-plant systems in Louisiana and impact of management practices on SSB activity and population are needed to fully develop this bio-based approach to improve Si nutrition in soil and plants.